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One ml of the bacterial suspensions was washed twice in Phosphate Buffered Saline (PBS, pH 7.2) at 5000 g, 4C, for 10 min and the optical density assessed by spectrophotometry at 600 nm
On examination, suprapubic tenderness is common along with external (perineal) tenderness and internal (levator muscle) tenderness/spasticity
Immunomodulation There are no recommended immunomodulatory therapies, but the following may be used on a patient-by-patient basis: Ciclosporin
No human timeline data exists for gut-specific applications
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